| Title | Probing the function of neuronal populations: combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging |
| Author | Sachiko Tsuda a, b, c, Michelle Z.L. Kee a, b, c, Catarina Cunha c, d, Jinsook Kim a, b, c, Ping Yan e, Leslie M. Loew e, George J. Augustine a, b, c, f |
| Affiliation(s) | Laboratory of Synaptic Circuitry, Program in Neuroscience and Behavioral Disorders, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, Singapore
A*STAR/Duke-NUS Neuroscience Research Partnership, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore
Marine Biological Laboratory, Woods Hole, MA 02543, USA Center for Neural Science, New York University, New York, NY 10003, USA
Richard D. Berlin Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA |
| Published | |
| Keyword | 460nm Optogenetic photostimulation collimated Optogenetics; Channelrhodopsin; Digital micromirror device; Voltage-sensitive dye imaging; Inhibitory circuitry; Cerebellum |
| Snippet | ... 2.4. Optogenetic photostimulation. Photostimulation was done with a digital micromirror device
(Mosaic, Andor), using a high-power (collimated power output: 1.5 W) light-emitting diode (LED,
Prizmatix). The peak emission wavelength of this LED was 460 nm (32 nm FWHM). .. |
| Abstract | Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical detection of resulting postsynaptic activity. Such technology allowed us to control the activity of cerebellar interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, their postsynaptic targets. This approach should substantially accelerate our understanding of information processing by populations of neurons within brain circuits. |
