Ultra High Power LEDs

(see [1] on drawing)

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Ultra High Power (UHP) Ultra High Power (UHP-T-EP) LEDs incorporate advanced electronics and optics designed for use in demanding Electrophysiology and Optogenetics applications. The UHP-T-EP (electrophysiology) features an ultra-high brightness single emitter LED (as opposed to LED array) optimized for both epifluorescence excitation and fiber coupled use. Compatible with Prizmatix’s Optiblock system, a single LED can be expanded to multi-wavelength or multi-output use with ease.

Sophisticated electronics eliminates virtually all EM/RF noise and may be used inside or near EP rigs without shielding. The UHP-T LED driver includes TTL and Analog input as standard features. The TTL input is ideal for fast switching with a rise/fall time of microseconds, exceeding the required millisecond speed of most Optogenetics applications. Analog input allows for software control of intensity using a 0-5v signal.

Available in Blue for Chr2 and CHETA, Green for Arch and ArchT, Red and Red-Amber for eNpHR3.0 and in UV/Violet for Uncaging.

The Beam Combiner can join two discrete UHP-LEDs into one output beam, which can then be coupled to a microscope or an optical fiber. For increased versatility, additional optical filters can be installed at the input or the output ports of the Beam Combiner.

The UHP-LED can be easily changed from direct microscope coupling to fiber coupled LED configuration, using this Fiber Coupler Adapator 

Prizmatix offers a wide range of standard and customized multi-mode silica / polymer fibers as well as ferrules for optogenetics research. A Y-shaped fiber bundle is especially useful, offering the option to activate two brain hemispheres simultaneously: a 1000μm or 1500μm fiber is coupled to the LED and connected to a rotary joint. From the joint’s other side emerge two thin fibers which can then be connected to two separate ferrules. The light from the LED is divided equally between the two hemispheres without any loss of power.

Prizmatix's Rotary Joint is specially designed for Optogenetics experiments with High NA detachable fibers equipped with FC connectors. Rotary Joint's extremely low friction and smooth swivel operation are demonstrated in this video - showing continuous rotation for over 30 seconds after a fingertip start-up. The torque required for free movement is very low thus reducing the Rotary Joint's behavioral effect on free moving animals, making it suitable for even the smallest rodents. Prizmatix Rotary Joint can be used with single or multiple output fibers simultaneously, enabling concurrent delivery of light to separate areas of the brain without loss of power or brightness. See more..

Rotary Joint

The Beam Switcher accessory allows for Prizmatix LEDs installed on a microscope to be used either as microscope epi-fluorescence illumination or illuminating a specimen via a fiberoptic probe.
Especially useful for in-situ Optogenetics studies, the Beam Switcher saves cost, space and allows maximum flexibility in experiment design. See more..

Beam switcher

The UHP-Mic-LED can be equipped with a 6 positions filter wheel at the beam output. This accessory is particularly useful for UHP-Mic-LED-White light source. See more ...

Filter Wheel PDF

Use these adaptors to connect Prizmatix LED light sources to the most widely used microscopes. Adaptors for epi-fluorescence ports of Nikon, Zeiss, Olympus and Leica microscopes are available.

The UHP-LEDs can be easily changed from direct microscope coupling to Liquid Light Gide (LLG) coupled using this Adaptor. This adaptor can be without difficulty assembled by the user on the UHP-LED output.
Please see video clip for more details.

The Prizmatix LLG collimator is an XYZ adjustable collimator for Liquid Light Guide (LLG) with adaptor for the epi-illumination port of fluorescence microscopes. This collimator can be equipped with Nikon, Olympus, Zeiss or Leica adaptors.

A standard C-mount thread adaptor (1-32 UN 2A thread), usually used to connect to trinocular microscopes.

Collimators are required to generate a parallel beam of light out of the naturally diverging light emission from an optical fiber.

Prizmatix collimator is designed to collimate High NA fibers (up to 0.53), such as Hard Polymer Clad fibers and Polymer Optical Fibers (POFs).

Collimator

Fiberoptics for Optogenetics

Optogenetics Fiber

Optogenetics Dual Fiber

Armored Optogenetics Fiber

Armored Optogenetics Fiber Dual

Pulser / PulserPlus

The Pulser device is TTL-Pulse-Generator for Optogenetics experiments.

Pulser provides an easy and inexpensive way to create trains of TTL pulses for Optogenetics activation directly from your computer via USB. It can work with Prizmatix Optogenetics-LEDs, Lasers or Shutters.

TTL-Out connector used for TTL pulses with sophisticated sequences (single or bi-phasic pulses as single pulse train or multiple groups of trains are possible).

Trigger-In connector is provided for synchronization of Optogenetics with other experiment events ( e.g. events created by video tracking software). Five different Trigger Operation Modes are provided.

Trigger-Out connector enables further activation of additional devices after pulse train was completed ( PulserPlus only)

The Pulser software enables the simple visual design of various TTL pulse trains. The software is available as a stand-alone Windows application, as well as web-based application running on Google’s Chrome browser, enabling the use of the Pulser on Mac and Windows platforms.

Pulser includes all necessary hardware, software and cables.  See more ...