Prizmatix offers a range of modules for optogenetics in-vitro experiments starting from simple plug-and-go
starter kits (Optogenetics LEDs) to modular Ultra High Power (UHP) LEDs enabling you to
create customized single or multi-wavelength set-ups to suit your application.
With speeds of better than millisecond-scale temporal precision, our LED light sources
are exellent for dopsin activation and silencing.
From light source to cannula and everything between,
Prizmatix can provide all the components necessary for
your research - including advice and guidance.
For Optogenetics In-Vivo and relevant accessories
click here
Ultra High Power (UHP)
Ultra High Power (UHP-T-EP) LEDs incorporate advanced electronics and
optics designed for use in demanding Electrophysiology and Optogenetics
applications. The UHP-T-EP (electrophysiology) features an ultra-high brightness
single emitter LED (as opposed to LED array) optimized for both
epifluorescence excitation and fiber coupled use.
Compatible with Prizmatix’s Optiblock system, a single LED can be expanded
to multi-wavelength or multi-output use with ease.
Sophisticated electronics eliminates virtually all EM/RF noise and may be
used inside or near EP rigs without shielding. The UHP-T LED driver includes
TTL and Analog input as standard features. The TTL input is ideal for fast
switching with a rise/fall time of microseconds, exceeding the required
millisecond speed of most Optogenetics applications. Analog input allows
for software control of intensity using a 0-5v signal.
Available in Blue for Chr2 and CHETA, Green for Arch and ArchT,
Red and Red-Amber for eNpHR3.0 and in UV/Violet for Uncaging.
The Beam Combiner can join two discrete UHP-LEDs into one output beam, which
can then be coupled to a microscope or an optical fiber. For increased versatility,
additional optical filters can be installed at the input or the output ports of the Beam Combiner.
Prizmatix offers a wide range of standard
and customized multi-mode silica / polymer fibers as well
as ferrules for optogenetics research.
A Y-shaped fiber bundle is especially useful,
offering the option to activate two brain hemispheres
simultaneously: a 1000μm or 1500μm fiber is coupled to the LED
and connected to a rotary joint.
From the joint’s other side emerge two thin fibers
which can then be connected to two separate ferrules. The light from the LED is
divided equally between the two hemispheres without any loss of power.
Prizmatix's Rotary Joint is specially designed for Optogenetics
experiments with High NA detachable fibers equipped with FC connectors.
Rotary Joint's extremely low friction and smooth swivel operation
are demonstrated in this video - showing continuous rotation for over 30 seconds
after a fingertip start-up.
The torque required for free movement is very low thus reducing the
Rotary Joint's behavioral effect on free moving animals, making it
suitable for even the smallest rodents.
Prizmatix Rotary Joint can be used with single or multiple output
fibers simultaneously, enabling concurrent delivery of light to separate
areas of the brain without loss of power or brightness.
See more..
The Beam Switcher accessory allows for Prizmatix LEDs installed on a microscope to be used
either as microscope epi-fluorescence illumination or illuminating a specimen via a fiberoptic probe.
Especially useful for in-situ Optogenetics studies, the Beam Switcher saves cost,
space and allows maximum flexibility in experiment design.
See more..
The UHP-Mic-LED can be equipped with a 6 positions filter wheel at the beam output.
This accessory is particularly useful for UHP-Mic-LED-White light source.
See more ...
Use these adaptors to connect Prizmatix LED light sources to the most widely used microscopes.
Adaptors for epi-fluorescence ports of Nikon, Zeiss, Olympus and Leica microscopes are available.
The UHP-LEDs can be easily changed from direct microscope coupling to
Liquid Light Gide (LLG) coupled using this Adaptor. This adaptor can be without difficulty
assembled by the user on the UHP-LED output.
Please see video clip for more details.
The Prizmatix LLG collimator is an XYZ adjustable collimator for Liquid Light Guide (LLG)
with adaptor for the epi-illumination port of fluorescence microscopes.
This collimator can be equipped with Nikon, Olympus, Zeiss or Leica adaptors.
Prizmatix reference photodiode
is useful in order to monitor LED source power over time in experiments
required exceptional stability over long time period.
The photodiode signal can be used to normalize measurements;
alternatively the photodiode signal can serve as a
feedback signal to stabilize the LED power.
See more..
Prizmatix provides diverse fiber optics solutions for optogenetics in-vivo. Made of
silica or polymer, these high NA fibers can be constructed to fit any research set-up
with various combinations of connectors, ferrules, core diameters and lengths.
Typical Optogenetics in-vivo fiber optics setup will include fiber patch cord from
Optogenetics-LED to Rotary Joint (P/N: Optogenetics-Fiber-1000), fiber to connect
the Rotary-Joint to cannula (P/N: Optogenetics-Fiber-500) or dual fiber for
bilateral activation (P/N: Optogenetics-Fiber-Dual-500). The later can be optimized
for mice (1.25mm ferrule) or to rat (2.5mm ferrule). In some setups this fiber can
be produced as Armored fiber with very light and flexible metal sheath. See more ...
Prizmatix Implantable Fiberoptic Cannulae / Ferrules
allow direct light stimulation of certain deep brain regions
in living animals.
Each Cannula consists of a Zirconia ferrule
accommodating a high NA Silica optical fiber, protruding from
the Zirconia ferrule at a desired length.
See more...
The Pulser device is an easy and inexpensive way
to create trains of TTL pulses for Optogenetics activation
directly from your computer via USB.
Pulser includes all necessary hardware, software and cables.
See more...
The software enables easy visual design of the various TTL pulse Trains and conditions. The pictures below show the Graphical User Interface (GUI). The upper right side of the GUI shows an example waveform with the legend of user definable parameters. The bottom pane shows the actual TTL waveform programmed with the user settings on the left side.
The software available as stand-alone Windows application as well as Web application running on Google Chrome browser enabling using of Pulser software on Mac or Windows computers without software installation.
Click here to run Pulser web application
!New: Now you can use Pulser also from Google Chrome browser on your Mac or Windows PC.
