The Prizmatix Optogenetics-LED-STSI module is specially designed to provide Optogenetics Stimulation / Silencing to the same neural structure in freely moving mammals. The device combines two LEDs of different colors into the same single output fiber. Each LED has independent power and switching control.
The wavelengths combination can suit widely used Stimulation and Silencing Optogenetics opsin pairs, such as Channelrhodopsin / Halorhodopsin (ChR2 / NpHR), ChR2 / eNpHR3.0 or Channelrhodopsin / Archaerhodopsin (ChR2 / ArchT). These opsins pairs can be stimulated / silenced by for example Blue / Lime-Green or Blue / Green wavelengths pairs. Other wavelengths are available upon request.
The Optogenetics-LED-STSI based system optionally enables bilateral activation / inhibition with single device and single one-channel rotary joint for significant cost saving and without compromising power at implant tip.
Key Features
- High Power density (>240 mW/mm²) at cannula's fiber tip following all fiberoptics connections and rotary joint.
- Unique large and powerful LEDs enable bilateral illumination with single LED and rotary joint for significant cost saving and without compromising power at implant tip.
- Extremely low torque LED-compatible rotary joint suitable for even the smallest animals
- Precisely adjustable power by 10 turns potentiometers, or electronically controlled by Analog input ports (0-5V)
- TTL and Analog Inputs are Opto-Isolated to eliminate ground-loops
- Instant warm up time. Rise / Fall time <10µs
Optogenetics Toolbox for Freely Moving Mammals Experiments
Prizmatix offers a wide range of standard and customized items comprising the Optogenetics Toolbox. The most useful items for optogenetics experiments in free-moving animals are:
- Optogenetics-LED-STSI
- Extremely low friction Rotary-Joint
- Fiber Optics Cannulae
- Single or Dual Fiber for bilateral activation
- Fiber patch cord
- Optional Pulser module with Windows software
For more information on items 2-6 please see Optional Accessories below
Common LED Colors:
| Opsin | LED Color | Peak wavelength range (nm) |
| UV- Violet | 385 - 410 | |
| ChR2
Channelrhodopsin |
Blue | 450 - 465 |
| Arch Archaerhodopsin |
Green | 515 - 525 |
| ArchT | Green | 515 - 525 |
| C1V1 | Green | 515 - 525 |
| NpHR Halorhodopsin |
Lime-Green | 540- 600 |
| eNpHR3.0 | Orange-Red | 600 - 630 |
Optical Specifications
| LED specifications | Units | Channel #1
|
Channel #2
|
| Peak wavelength range | |||
| Spectrum FWHM | nm | ||
| Optical ouput power values from optical fiber connected directly to Optogenetics-LED.
Useful for in-vitro or fixed preparate applications: |
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| Optical Power from 200µμm NA0.66 fiber (1m) | mW | ||
| Optical Power from 250μm NA0.66 fiber (1m) | mW | ||
| Optical Power from 500μm NA0.63 fiber (1m) | mW | ||
| Optical Power from 1000μm NA0.63 fiber (1m) | mW | ||
| Optical output power values from cannula of full Optogenetics system comprising Optogenetics-LED, fiber patch cord, Rotary-Joint, Dual/Single fiber and cannula inplant: | |||
| Optical Power from 200μm cannula | mW | ||
| Optical Power from 250μm cannula | mW | ||
| Optical Power from 500μm cannula | mW | ||
| Irradiance from 200μm cannula | mW/mm² | ||
Electrical Specifications
General Specifications
Optional AccessoriesFor more details on optional accessories please see: https://www.prizmatix.com/Optogenetics/Prizmatix-in-vivo-Optogenetics-Toolbox.htm
Optogenetics Implantable Cannulae
Single/Dual Fibre
Fiber Patch CordsPrizmatix offers a wide range of standard and customized multi-mode silica / polymer fibers as well as ferrules for optogenetics research. A Y-shaped fiber bundle is especially useful for activating two brain hemispheres simultaneously: a 1000µm or 1500µm fiber is coupled to the LED and connected to one side of the Rotary Joint. From the Joint's other side emerge two thin fibers which can then be connected to two separate ferrules: the light from the LED is divided equally between the two hemispheres without any loss of power. PulserOptogenetics Pulser is a programmable TTL pulse train generator for pulsing LEDs, lasers and shutters used in Optogenetics activation. The Pulser device comes with user friendly software that enables easy visual setup of numerous pulse train configurations. See Pulser product details 
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