Optogenetics-LED-Dual


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Engineered for scaling Optogenetics experiments, the Optogenetics-LED-Dual light source features two independent fiber-coupled LED channels each equipped with independent power and switching control.

This versatile system accommodates two LEDs with a wavelength combination that can suit widely used Stimulation or Silencing Optogenetics opsins, such as Channelrhodopsin ChR2, Halorhodopsin NpHR, eNpHR3.0, Archaerhodopsin Arch, ArchT etc. The available wavelengths for each channel span the Violet, Blue, Green, Yellow, Red, Deep-Red, and Near-IR with the flexibility to customize any wavelength combination upon request.

Each channel in the Optogenetics-LED-Dual system allows for bilateral activation/inhibition, ensuring substantial cost savings without compromising power at the implant tip.

Optogenetics-Toolbox-in-vivo

Optogenetics-LED-Dual

Optogenetics-LED-User-Manual

Key Features

High Power density (at Blue >380 mW/mm²) at implant tip following Rotary-Joint and all fiber connections.
Unique large and powerful LEDs enable bilateral illumination with single LED and Rotary-Joint for significant cost saving and without compromising power at implant tip.
Extremely low torque LED-compatible Rotary-Joint suitable for even the smallest animals
Precisely adjustable power by 10 turns potentiometers (separately for each channel)
Opto-Isolated TTL Inputs for fast switching (separately for each channel)
Opto-Isolated Analog Inputs for external power control (separately for each channel)
Instant warm up time. Rise / Fall time <10 μs

Optogenetics Toolbox for Freely Moving Mammals Experiments

Prizmatix offers a wide range of standard and customized items comprising the Optogenetics Toolbox. The most useful items for optogenetics experiments in freely moving mammals are:

Optogenetics-LED-Dual
Extremely low friction Rotary-Joint
Fiber Optics Cannulae
Single or Dual Fiber for bilateral activation
Fiber patch cord
Pulser for generation of TTL pulse trains

Optogenetics-LED-Dual-System — Click to enlarge

Common LED Colors:

Opsin	LED Color	Peak wavelength range (nm)
	UV- Violet	385 - 410
ChR2 Channelrhodopsin	Blue	450 - 465
Arch Archaerhodopsin	Green	515 - 525
ArchT	Green	515 - 525
C1V1	Green	515 - 525
NpHR Halorhodopsin	Lime-Green	540- 600
eNpHR3.0	Orange-Red	600 - 630

Optical Specifications

LED specifications

Units

Channel #1 LED

Channel #2 LED

Peak wavelength range

Spectrum FWHM

Optical ouput power values from optical fiber connected directly to Optogenetics-LED.
Useful for in-vitro or fixed preparate applications:

Optical Power from 200μm NA0.66 fiber (1m)

Optical Power from 250μm NA0.66 fiber (1m)

Optical Power from 500μm NA0.63 fiber (1m)

Optical Power from 1000μm NA0.63 fiber (1m)

Optical output power values from cannula of full Optogenetics system comprising Optogenetics-LED, fiber patch cord, Rotary-Joint, Dual/Single fiber and cannula inplant:

Optical Power from 200μm cannula

Optical Power from 250μm cannula

Optical Power from 500μm cannula

Irradiance from 200μm cannula

mW/mm²

Opsin #1

Opsin #2

Output Data

λ = 314

Channel #1 = 0.001

Channel #2 = 0.000

Wavelength (nm)

Electrical Specifications

Digital modulation input		Optically isolated TTL
Connector for TTL and Analog input		BNC
Digital modulation frequency	Hz	DC-30000
Rise / Fall time (10% - 90%)	µs	<10
Analog input voltage range	V	0-5
Input Voltage	V	12
Power Adaptor Input		85-264 VAC, 47-63Hz,1.5A

General Specifications

Operation temperature range	°C	10-35
Storage Temperature range	°C	-10-35
Operating relative humidity Non condensing	%	<90
Dimensions (L x W x H)	mm	197 x 241 x 80
Weight	g	750
Power Adaptor Dimensions (L x W x H)	mm	125 x 50 x 31.5
Power Adaptor Weight	g	270
Power Adaptor Safety
Fan Noise	dBA	28.4

Optional Accessories

For more details on optional accessories please see:

https://www.prizmatix.com/Optogenetics/Prizmatix-in-vivo-Optogenetics-Toolbox.htm

Rotary Joint

Prizmatix Rotary Joint is especially designed for Optogenetics experiments with High NA fibers equipped with FC connectors. Rotary Joint very low friction and smooth swivel operation are demonstrated in this video - showing continuous rotation for over 30 seconds after a fingertip startup. The torque required for free movement is very low thus reducing the Rotary Joint's behavioral effect on freely moving animals.
Prizmatix Rotary Joint can be used with large core input optical fiber connected to several thin output fibers simultaneously. This allows concurrent delivery of light to separate areas of the brain without loss of power due to the fiber split.
See Rotary Joint product details

Rotary Joint

Optogenetics Implantable Cannulae

Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals. Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from the Zirconia ferrule at a desired length.
See Optogenetics Implantable Cannulae product details

Cannula

Single/Dual Fibre

Prizmatix provides a full solution to the optogenetics in vivo and in vitro fiber optics. Made of silica or polymer fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. Some examples are Y-shaped fibers for simultaneous in-vivo stimulation of two hemispheres, and fibers with stainless steel tip with protruding bare fibers for in-vitro activation of brain slices under a microscope.
See Single/Dual Fibre product details

Fiberoptics for Optogenetics

Fiber Patch Cords

Prizmatix offers a wide range of standard and customized multi-mode silica / polymer fibers as well as ferrules for optogenetics research. A Y-shaped fiber bundle is especially useful for activating two brain hemispheres simultaneously: a 1000µm or 1500µm fiber is coupled to the LED and connected to one side of the Rotary Joint. From the Joint's other side emerge two thin fibers which can then be connected to two separate ferrules: the light from the LED is divided equally between the two hemispheres without any loss of power.

Pulser

Optogenetics Pulser is a programmable TTL pulse train generator for pulsing LEDs, lasers and shutters used in Optogenetics activation. The Pulser device comes with user friendly software that enables easy visual setup of numerous pulse train configurations. See Pulser product details

Pulser